>>BOT 402 NOTES (2023-24 & 2024-25)

BOTANY
PAPER - BOT 402 MOLECULAR BIOLOGY AND BIOTECHNOLOGY:-

Unit-I 

Lecture - 1 Molecular Biology: The discovery of DNA, evidences indication, DNA as the genetic material, DNA and its types (A, B and Z DNA), closed super coiled DNA, denaturing and renaturing of DNA, hybridization:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/molecular-biology-discovery-of-dna.html

Lecture - 2 DNA Replicaton: mechanism, enzymes, evidences in favour of semi conservative replication:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/dna-replication-mechanism-enzymes.html

Unit-II

Lecture - 3 RNA: Structures, types, transcription, control at initiation, control at termination, attenuation, heterogenous RNA processing, capping and tailing:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/rna-structures-types-transcription.html

Lecture - 4 Techniques employed in recombination DNA technology, isolation and purification of DNA, Electrophoresis (Gel, Agarose), DNA sequencing, Southern and Northern blotting, PCR and its application:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/techniques-employed-in-recombination.html

Lecture - 5 Gene structure and function, lac operon, trytophan operon:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/gene-structure-and-function-lac-operon.html

Unit-III

Lecture - 6 Biotransformation; Production of useful compounds through cell-culture, Biofermentor:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/biotransformation-production-of-useful.html

Lecture - 7 Vectors, plasmids, cosmids, Cloning strategies:-

https://mscbotanysemivmgsubikaner.blogspot.com/2025/08/lecture-8-gene-transfer-techniques.html

Lecture - 8 Basic concepts about cDNA, gene and genomic library:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/basic-concepts-about-cdna-and-genomic.html

Lecture - 9 Applications of Recombinant DNA Technology:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/transgenic-plants-and-methods.html

Unit-IV

Lecture - 10 Genetic Engineering and its principles:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/genetic-engineering-and-its-principles.html

Lecture - 11 Gene transfer:-

https://mscbotanysemivmgsubikaner.blogspot.com/2025/08/lecture-9-gene-transfer-in-plants-agro.html

Lecture - 12 Transgenic plants: Methods, Production, Application and Use:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/transgenic-plants-and-methods.html

Lecture - 13 Applications of Recombinant DNA Technology:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/transgenic-plants-and-methods.html

Unit-V

Lecture - 14 Biotechnology: Concept and Scope:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/biotechnology-concept-and-scope-of.html

Lecture - 15 Plant tissue culture:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/plant-tissue-culture-anther-and-pollen.html

Lecture - 16 Micropropagation, callus culture, Anther and Pollen culture:-

https://mscbotanysemivmgsubikaner.blogspot.com/2025/08/lecture-12-micropropagation-callus.html

Lecture - 17 Protoplast culture: Isolation, purification and culture, protoplast fusion, Cybrids, Somatic hybrids.:-