>>BOT 402 NOTES

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PAPER - BOT 402 MOLECULAR BIOLOGY AND BIOTECHNOLOGY:-

Unit-I 

Lecture - 1 Molecular Biology: The discovery of DNA, evidences indication DNA as the genetic material, DNA and its types ( A,B and Z DNA), closed super coiled DNA, denaturing and renaturing of DNA, hybridization:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/molecular-biology-discovery-of-dna.html

Lecture - 2 DNA Replicaton: mechanism, enzymes, evidences in favour of semi conservative replication:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/dna-replication-mechanism-enzymes.html

Unit-II

Lecture - 3 RNA: Structures, types, transcription, control at initiation , control at termination, attenuation, heterogenous RNA processing, capping and tailing:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/rna-structures-types-transcription.html

Lecture - 4 Techniques employed in recombination DNA technology, isolation and purification of DNA gel electrophoresis (Gel, Agarose), DNA sequencing, Southern and Northern blotting, PCR and its application:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/techniques-employed-in-recombination.html

Lecture - 5 Gene structure and function, lac operon, trytophan operon:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/gene-structure-and-function-lac-operon.html

Unit-III

Lecture - 6 Biotransformation; Production of useful compounds through cell-culture:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/biotransformation-production-of-useful.html

Lecture - 7 Vectors: plasmids and cosmids, cloning strategies:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/vectors-plasmids-and-cosmids-cloning.html

Lecture - 8 Basic concepts about cDNA and genomic-library:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/basic-concepts-about-cdna-and-genomic.html

Lecture - 9 Application of recombinant DNA technology:-

 https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/application-of-recombinant-dna.html

Unit-IV

Lecture - 10 Genetic engineering and its principles, gene - transfer:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/genetic-engineering-and-its-principles.html

Lecture - 11 Transgenic plants and methods, production, application and use:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/transgenic-plants-and-methods.html

Lecture - 12 Importance of genetic engineering:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/importance-of-genetic-engineering.html

Unit-V

Lecture - 13 Biotechnology: concept and scope of biotechnology:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/biotechnology-concept-and-scope-of.html

Lecture - 14 Plant tissue-culture, anther and pollen culture, callus-culture:-

https://mscbotanysemivmgsubikaner.blogspot.com/2024/05/plant-tissue-culture-anther-and-pollen.html

Lecture - 15 Protoplast culture: Isolation, purification, culture and fusion: Cybrids and hybrids:-